#Per2 snapgene how to#
#Per2 snapgene update#

Once we identify the gene, we can easily annotate it by making a custom feature in Snapgene. This saves us time as we are often analyzing deposits with many plasmids that contain similar features and it allows you to more quickly identify the same feature in other plasmids. Once we’ve identified the features within a plasmid, we next verify that our sequencing results match sequence information contributed by the depositor, published backbone sequences, and any known sequences for the insert. The Snapgene desktop software allows us to quickly align multiple sequences at the same time. Using Snapgene for additional verification experiments: Primer and restriction digest design This means we can easily compare and analyze any differences between our sequence results and the reference sequences. Some regions of plasmids are difficult to sequence by NGS. These can include GC-rich and repetitive regions. These regions require additional verification experiments that can be partially designed using the Snapgene desktop software. If a plasmid contains a region with a high amount of guanine and cytosine nucleotides, we might need to send it for Sanger sequencing to verify the sequence within that region. We have designed many primers for Sanger sequencing at Addgene. By using Snapgene’s “Import primers from a list” function, we can detect all of the primers we have designed that will anneal to the plasmid sequence. We can then quickly choose which primers we should use to further verify the difficult regions and send the plasmid off for Sanger sequencing.

#Per2 snapgene how to#

By using the “Simulate agarose gel” option with the desktop version of Snapgene, we can predict the resulting band sizes when digesting the plasmid sequence with one or more enzymes.Ĭlick here to learn how to run an agarose gel If a repetitive region is present in the plasmid, we might need to perform a restriction digest to confirm the size of the plasmid and the insert.

#Per2 snapgene update#

We’ve worked with Snapgene to update our website and enable you to analyze sequencing results for plasmids in the repository at a glance. Our Snapgene-powered plasmid maps and sequences use the same feature detection software as the desktop version of Snapgene. When you download a Snapgene file directly from our website, you can open it with either the Snapgene viewer or Snapgene Desktop programs to analyze the sequence data in depth.